Course Description: Electron microscopes are valuable research tools that have provided a wealth of information about cell structure and function. Using the new transmission electron microscopy (TEM) laboratory in the Life Sciences Center, this course provides an introduction to cellular ultrastructure and to techniques through which it can be studied. Students will learn techniques of tissue fixation, embedding, sectioning (ultramicrotomy), and staining, as well as use of the TEM to visualize and photograph specimens. Microscopic structure of cells and tissues also will be studied using prepared specimens. This course is primarily designed for students interested in doing research in biology, biochemistry, or neuroscience.

Prerequisites: Biology 152L and 153L, and permission of Prof. Blackburn.

Lectures: Mondays, 11:00-11:50 PM

Labs: Wednesdays, 1:20 - 3:55 PM, 331 LSC

Course Personnel: Dr. Daniel Blackburn, with Ms. Ann Lehman (Asst. Director of the EM Facility)

Note: In addition to the formal laboratory period, students need to schedule a second full afternoon per week, as well as to arrange blocks of time to practice the several techniques taught. Because of the nature of the course, enrollment is limited, and students contemplating registration should contact Dr. Blackburn and / or Ms. Lehman well in advance to discuss their interests.

D. Blackburn's home page